Due to the complete absence of information regarding the film's narrative, characters, or themes, it is not possible to determine if LGBTQ+ characters or themes are present. Consequently, an evaluation of their portrayal and net impact cannot be made, leading to an N/K assessment.

There is insufficient information regarding the film's plot or characters to assess its portrayal of transsexual individuals or themes. Without specific details, a determination of positive, negative, or neutral impact cannot be made, as no relevant character arcs or story plots are known.

Due to the limited information available for "Press Start!, 2024," specifically the absence of plot details or character actions, it is not possible to determine if any female character engages in and wins direct physical combat against male opponents.

Without information on source material, previous installments, or historical context for 'Press Start!, 2024', it is impossible to determine if any characters were canonically established as a different gender prior to this film.

The film "Press Start!, 2024" is presented without information regarding any pre-existing source material or historical basis. Therefore, there is no established canonical race for its characters to compare against the on-screen portrayals, making it impossible to determine if a race swap occurred.