Steven Universe offers an overwhelmingly positive portrayal of LGBTQ+ characters and themes. It prominently features diverse queer relationships, such as Ruby and Sapphire's marriage, and explores non-binary identity through Stevonnie, consistently affirming love, identity, and acceptance as core strengths.

Steven Universe positively portrays gender diversity through its non-binary characters. Stevonnie and Shep, who use they/them pronouns, are depicted with dignity, agency, and respect, embodying the show's affirming stance on varied gender identities. The narrative consistently celebrates self-acceptance and challenges traditional gender norms.

The show features Bismuth, a powerful Gem, who engages in direct physical combat with Steven, a male character. Bismuth consistently demonstrates superior strength and skill, repeatedly defeating Steven in hand-to-hand and melee weapon exchanges during their fight.