The Blacklist features Aram Mojtabai, a main character who explores his sexuality and engages in a same-sex relationship. His journey is depicted with dignity and complexity, presented as a natural part of his personal growth rather than a source of mockery or misery. The show maintains a respectful and empathetic stance toward his queer identity.

The Blacklist features a central character, Raymond Reddington, revealed to be a trans woman, Katarina Rostova, who transitioned to protect her daughter. This character is consistently depicted with dignity, complexity, and agency, with her transition framed as a profound act of love and sacrifice. A minor character, Elodie Radcliffe, is also a trans woman, whose identity is accepted by her partner. The net impact is primarily positive.

While extremist groups claiming Islamic affiliation are sometimes antagonists, the show often features sympathetic Muslim characters and distinguishes between individual faith and radicalism. The narrative positions the audience to condemn the extremism and its perpetrators, not the religion itself.