The Dragon Prince features multiple LGBTQ+ characters and relationships, including lesbian queens, a non-binary scholar, and a prominent lesbian couple whose relationship is central to the plot. These characters are portrayed with dignity, complexity, and agency, and their identities are normalized and affirmed within the narrative, contributing to a strongly positive overall impact.

The Dragon Prince features Kazi, a non-binary Sunfire elf healer, whose identity is portrayed with dignity and acceptance. Kazi is a respected and competent character, and their pronouns are consistently used without question, affirming their identity as a normal and integrated aspect of their person within the story.

The show features several prominent female characters who are skilled warriors and combatants. Multiple scenes depict these characters engaging in and winning direct physical fights against one or more male opponents, utilizing melee weapons, martial arts, and hand-to-hand combat.