The Magic School Bus is an educational children's animated series primarily focused on science. The show does not include any discernible LGBTQ+ characters or themes, as its narrative scope is centered on scientific exploration and learning for a young audience.

The Magic School Bus is an educational children's animated series focused on science. It does not feature any identifiable transsexual characters or explore related themes, as its narrative is entirely dedicated to scientific concepts and adventures.

The Magic School Bus is an educational children's animated series focused on science exploration and adventure. It does not feature any scenes of physical combat between characters, male or female, and therefore no instances of female characters defeating male opponents in direct physical confrontation.

The 1994 animated series "The Magic School Bus" faithfully adapts the characters from the original book series. All established major characters, such as Ms. Frizzle, Arnold, and Ralphie, retain their canonical genders from the source material.

The 1994 animated series "The Magic School Bus" adapted characters from the original book series. All main characters retained their established racial depictions from the source material, with no changes in race for any character.