This animated film does not feature any identifiable LGBTQ+ characters or themes. The storyline centers on a group of sisters, their family dynamics, and a magical adventure, with all relationships and character identities presented as heterosexual. Therefore, the film has no net impact on LGBTQ+ portrayal.

This animated film does not feature any identifiable transsexual characters or themes. The story centers on the adventures of twelve princesses and their magical dancing, with no elements pertaining to transgender identity or experiences present in the plot or character arcs.

The movie does not contain any action or adventure elements.

This film is an adaptation of the fairy tale "The Twelve Dancing Princesses." All major characters, including the twelve princesses and the male protagonist, maintain their canonical genders from the source material. No established characters undergo a gender change.

The film adapts a Brothers Grimm fairy tale, which traditionally features characters of European descent. All main characters in the 2006 animated film are depicted as white, consistent with the implied race of the source material and prior Barbie character portrayals. No character established as one race was portrayed as a different race.