The Owl House offers a profoundly positive and affirming portrayal of LGBTQ+ characters and themes. It features a central bisexual protagonist and lesbian love interest, alongside a prominent non-binary character, whose identities are normalized, celebrated, and integral to the show's narrative and emotional core.

The Owl House features non-binary characters, Raine Whispers and Masha, whose identities are depicted with dignity and normalcy. They are respected, complex individuals whose gender identity is not a source of conflict or mockery. The show's overall portrayal is affirming, integrating these characters positively into the narrative without their identity being a tragic or problematic element.

The show features Eda Clawthorne, a powerful witch, who repeatedly engages in and wins close-quarters physical fights against multiple male opponents, often utilizing her staff as a melee weapon.