The 1998 anime series TRIGUN does not feature any identifiable LGBTQ+ characters or themes. The narrative primarily focuses on Vash the Stampede's journey, philosophical conflicts, and action sequences, with no explicit or implicit LGBTQ+ representation present in the storyline or character arcs.

The film TRIGUN (1998) does not feature any identifiable transsexual characters or themes. The narrative primarily focuses on the adventures of Vash the Stampede and his companions in a futuristic, desert world, without exploring gender identity in this specific context.

The series features Meryl Stryfe and Milly Thompson as prominent female characters. Meryl primarily uses a small firearm for defense. Milly, while physically strong, achieves her combat victories using a large, non-lethal stun gun. Neither character is depicted winning against male opponents in direct hand-to-hand or melee weapon combat.

The 1998 TRIGUN anime adapts characters directly from the original manga by Yasuhiro Nightow. All major characters, such as Vash the Stampede, Meryl Stryfe, and Nicholas D. Wolfwood, maintain their established genders from the source material.

The 1998 anime adaptation of TRIGUN faithfully portrays its characters as established in the original manga. There are no instances where a character canonically or widely established as one race is depicted as a different race in this series.