Tuca & Bertie features a diverse cast that includes several LGBTQ+ characters. These individuals are depicted with complexity and agency, their identities integrated naturally into the show's world. The series consistently portrays queer lives and relationships in an affirming and respectful manner, contributing to its progressive themes.

Tuca & Bertie offers a positive portrayal of trans and non-binary identities. The series features characters like Dr.aca, a non-binary therapist, depicted with dignity and complexity. The narrative consistently affirms diverse identities, integrating them naturally into the characters' lives without relying on harmful stereotypes. This approach ensures a supportive and validating overall impact.

The series emphasizes chosen family bonds and normalizes diverse relationship structures and sexual freedom, presenting a progressive view of family life that moves away from traditional norms.