The series features multiple LGBTQ+ characters whose identities and relationships are depicted with dignity, complexity, and agency. Despite one gay antagonist, the overall portrayal is overwhelmingly positive, showcasing queer love and lives as valid and integral to the heroic arcs without judgment or negative framing.

The TV series 'Van Helsing' features Lucky, a trans woman, as a recurring character. Her identity is portrayed with dignity and respect, and the narrative actively counters prejudice against her. She is depicted as a capable survivor and has a positive romantic relationship, contributing to an overall affirming representation.

The show features multiple female characters, including Vanessa Van Helsing and Scarlett Harker, who engage in and win close-quarters physical combat against male opponents. Vanessa uses her enhanced strength and speed, while Scarlett relies on martial arts and melee weapons to defeat multiple adversaries.

The show reimagines the iconic vampire hunter Van Helsing, traditionally established as male in source material, as a female protagonist named Vanessa Van Helsing, constituting a clear gender swap.

The series frequently employs Christian themes of good versus evil, a chosen one with a divine purpose, and demonic forces. Characters who maintain Christian faith are generally depicted sympathetically, finding strength and moral guidance in their beliefs amidst a post-apocalyptic world. The narrative often aligns the fight against vampires with a righteous, divinely-backed struggle.