Weeds features LGBTQ+ characters like Jill Price-Gray, whose lesbian relationship is normalized, and Andy Botwin, whose sexual fluidity is explored. While Andy's experiences are sometimes comedic, the show integrates these identities without overt affirmation or denigration, resulting in a largely neutral portrayal where queer identity is present but not central to the narrative's core message.

The show features a character, Kevin, who transitions to Pam. While some characters, notably Celia, react with transphobia and mockery, Pam's personal journey of self-discovery and living authentically is also depicted. The portrayal balances problematic reactions from others with the character's agency, resulting in a largely neutral net impact.

The series frequently satirizes the hypocrisy and superficiality of Christian-identifying characters and institutions in suburban settings. It portrays adherence to Christian values as often a facade for immoral behavior or as a source of social conformity rather than genuine faith, without offering significant counterbalancing positive portrayals.