The film does not feature identifiable transgender characters or themes. The narrative contains no elements that depict or engage with transsexual identity, resulting in no overall impact on this specific aspect.

The film features female characters who participate in survival scenarios against zombies. No scenes depict a female character achieving victory in close-quarters physical combat against one or more human male opponents through skill, strength, or martial arts.

The film adapts characters from the original manga series. All main characters retain their established genders from the source material. No instances of gender swapping are present in this adaptation.

The animated characters in Zom 100: Bucket List of the Dead retain their original visual designs from the Japanese manga source material. The race of voice actors in an English dub does not alter the on-screen racial depiction of the animated characters. No character's race was changed from its established canon.