All About My Mother features multiple central LGBTQ+ characters, including transgender women and lesbian women, who are portrayed with profound dignity, complexity, and agency. Despite tragic events, the film consistently maintains an empathetic stance, celebrating resilience, love, and the formation of unconventional, supportive families, resulting in a deeply affirming net impact.

The film "All About My Mother" portrays transsexual characters with significant depth and empathy. Agrado is a resilient and dignified figure, while Lola is a complex, flawed individual whose identity is explored without judgment. The narrative affirms the worth and humanity of these characters, integrating their stories into a broader exploration of love, loss, and chosen family.

The film portrays Sister Rosa, a nun, with profound empathy and respect, highlighting her immense compassion and self-sacrifice for others, even as she faces personal struggles and judgment. While acknowledging the complexities and occasional hypocrisy within religious institutions or adherents, the narrative ultimately affirms the virtues and dignity found in her faith-driven actions.