No transsexual characters or themes appear in the film. The narrative focuses on historical events of exploration and colonization without addressing gender identity transitions or related motifs.

Female characters including Beatriz Barbosa de Magalhães and Queen Juana appear in supporting roles but do not participate in any depicted physical combat. The film portrays colonial violence through aftermath and implication rather than onscreen fights, with no instances of women defeating male opponents in close-quarters battle.

The film depicts historical figures like Ferdinand Magellan and Beatriz Barbosa with actors matching their documented genders, showing no gender swaps from historical canon.

The film casts actors whose racial backgrounds align with the historical figures' established ethnicities: European characters by white actors of European descent, and indigenous Filipino characters by Filipino actors. No instances of race swaps occur.