Alpha and Omega 2: A Howl-iday Adventure does not feature any identifiable LGBTQ+ characters or themes. The narrative centers on a heterosexual wolf couple and their pups, focusing on family-friendly holiday adventures without incorporating queer representation or related storylines.

The animated children's film, Alpha and Omega 2: A Howl-iday Adventure, does not feature any identifiable transsexual characters or themes. The narrative centers on a family of wolves and their pups, with no elements related to gender identity or transition present in the story or character arcs.

The film features female wolf characters like Kate who participate in group conflicts and defensive actions. However, there are no distinct scenes where a female character is shown to individually defeat one or more male opponents in direct, close-quarters physical combat through superior skill or strength.

All returning characters from the original film maintain their established genders. New characters introduced in this sequel do not constitute gender swaps as they lack prior canonical gender definitions.

The film features anthropomorphic wolf characters. As animals, these characters do not possess human racial characteristics, making the concept of a 'race swap' inapplicable to their portrayal.

Not depicted in the film.