Appleseed (2004) does not feature any identifiable LGBTQ+ characters or themes. The narrative is centered on science fiction action and political intrigue in a post-apocalyptic world, with no elements related to queer identity present in the story.

The film "Appleseed, 2004" does not feature any identifiable transsexual characters or themes. The narrative focuses on post-war society, cyborgs, and the future of humanity, without engaging with transgender identity or experiences. Therefore, there is no portrayal to evaluate within the scope of this rubric.

The film features Deunan Knute, a highly skilled female soldier who engages in numerous combat scenarios against male opponents. However, her victories are consistently achieved through the use of firearms and tactical maneuvers, rather than direct hand-to-hand combat, martial arts, or melee weapon engagements.

The 2004 film "Appleseed" adapts characters from the original manga. The main protagonists, Deunan Knute and Briareos Hecatonchires, maintain their established genders from the source material in the film adaptation.

The 2004 Japanese CGI anime film "Appleseed" faithfully adapts the visual character designs from the original manga by Masamune Shirow. There is no instance where a character canonically established as one race in the source material is portrayed as a different race in the film.