Barbie: Spy Squad is an animated children's film that focuses on adventure and friendship. There are no discernible LGBTQ+ characters or themes present in the movie's plot or character arcs. Consequently, the film's net impact on LGBTQ+ portrayal is N/A, as it does not engage with these identities or issues.

Barbie: Spy Squad is an animated children's film centered on adventure and friendship. It does not feature any identifiable transsexual characters or themes, nor does its narrative touch upon gender identity in a way that would be relevant to this evaluation framework.

The film focuses on Barbie, Teresa, and Renee as gymnasts turned secret agents. Their missions primarily involve stealth, acrobatics, and the use of gadgets to outsmart or evade antagonists. The main antagonist is female. There are no clear instances where female characters engage in and win direct physical combat against male opponents.

The film features Barbie and her friends in an original spy adventure. All main and supporting characters are either new creations for this specific story or maintain their established gender from within the Barbie franchise. There are no instances of characters being adapted from prior source material or history with a changed gender.

This animated film features established Barbie characters like Barbie, Teresa, and Renee, who maintain their historically consistent racial portrayals. No characters canonically established as one race are depicted as a different race in this installment.