The film features Borat's character making homophobic comments and expressing outdated views on gender and sexuality. These elements are primarily used for satirical purposes to expose prejudice and ignorance, rather than to endorse harmful stereotypes or focus centrally on LGBTQ+ identity. The overall portrayal is incidental, neither affirming nor denigrating LGBTQ+ people.

The film satirizes hypocrisy and extremism within certain Christian groups, particularly those intertwined with political conspiracies. However, it also features genuinely compassionate and moral Christian characters, framing their faith positively and contrasting it with Borat's bigotry.

The film actively combats antisemitism by portraying Jewish individuals with dignity and warmth, using Borat's initial prejudice as a vehicle to expose and condemn bigotry, ultimately showing his growth and challenging his stereotypes.