Enola Holmes defeats a male intruder in close-quarters hand-to-hand combat by pinning and choking him after a prolonged fistfight. She participates in additional physical confrontations during the story.

Professor Moriarty, canonically male in Arthur Conan Doyle's Sherlock Holmes stories, is portrayed by female actress Sharon Duncan-Brewster.

Dr. Watson, canonically white in Arthur Conan Doyle stories, is played by South Asian actor Himesh Patel. Moriarty, also canonically white, is played by Black actress Sharon Duncan-Brewster.

The film centers on Enola's wedding to Tewkesbury, with her initial doubts about losing independence and her Holmes identity, her brother's opposition to the marriage as restrictive, and her mother's feminist encouragement of autonomy; the story resolves with a supportive partnership where Enola keeps her name and they marry after confronting family legacies, framing marriage as compatible with but secondary to personal freedom and equality.