There is no available information regarding the portrayal of LGBTQ+ characters or themes in 'Fountain of Youth (2025)'. The existing details focus on the film's plot, cast, and production, without any discussion of LGBTQ+ representation. Therefore, a net impact assessment is not applicable.

Based on all available information, 'Fountain of Youth' (2025) does not appear to include or portray transsexual characters or explore themes of gender and sexual transformation. The film's narrative centers on an action-adventure quest for immortality, with no documented representation of transsexual identities or issues. For example, the plot focuses on two estranged siblings searching for a mythical fountain, without any mention of transsexual characters.

The film is an original story featuring new characters created specifically for it. There is no pre-existing source material or historical record to establish canonical genders for any character. Therefore, no character's on-screen gender differs from an established prior gender.

The film's source material is fictional and does not specify the race or ethnicity of its characters. Consequently, the diverse casting choices for major roles do not constitute race swaps, as there was no prior established race for any character to deviate from.