The film's portrayal of LGBTQ+ themes is primarily negative, utilizing gay stereotypes for comedic discomfort and employing homophobic slurs and threats of sexual assault as sources of intimidation and ridicule. There are no affirming depictions of LGBTQ+ characters or identities; instead, queer identity is largely presented as a source of mockery or degradation.

The film features characters whose gender presentation is used as a source of shock and comedic disgust for the protagonists. These portrayals rely on problematic stereotypes, presenting gender non-conformity as a punchline and eliciting negative reactions from the main characters, contributing to a net negative impact.

The film portrays characters who use Christian rhetoric to justify bigotry and torture, such as the antagonist Ron Fox. However, the narrative clearly condemns these characters and their actions, positioning the audience to view their hypocrisy and prejudice as wrong, thereby satirizing the misuse of faith.

The film strongly condemns Islamophobia and anti-Arab prejudice through its narrative. It portrays characters who are victims of such bigotry with sympathy, highlighting the absurdity and harm of these prejudices.

Jewish college students are depicted in a positive light, offering genuine help and hospitality to Harold and Kumar without judgment. Their portrayal is brief but consistently friendly and supportive.