The film 'My Little Pony: Equestria Girls' does not feature any identifiable LGBTQ+ characters or themes. The narrative centers on platonic friendships and heterosexual crushes, with no explicit queer representation present in its plot or character arcs.

The film 'My Little Pony: Equestria Girls' does not feature any identifiable transsexual characters or themes. The narrative focuses on friendship, magic, and high school challenges in an alternate human world, without engaging with transgender identity.

The movie does not contain any action or adventure elements.

The main characters from My Little Pony: Friendship Is Magic, originally female ponies and a male dragon, are depicted as female human-like teenagers and a male dog, respectively. Their genders remain consistent with their established portrayals in the source material.

The film adapts pony characters into human-like forms. As the original characters are ponies, they do not possess a human race in the source material. Therefore, their depiction with various skin tones in human form does not constitute a change from one established human race to another.