PAW Patrol: Mighty Pups is an animated children's film that centers on the heroic adventures of a group of rescue pups. The narrative does not include any identifiable LGBTQ+ characters, themes, or storylines, aligning with its target demographic and genre.

PAW Patrol: Mighty Pups is a children's animated film centered on a team of puppies with superpowers. The narrative does not include any identifiable transsexual characters, themes, or related plot points, resulting in no depiction to evaluate.

The film features female characters Skye and Everest, who are part of the PAW Patrol and gain superpowers. Their actions involve using these powers (wind and ice, respectively) to solve problems and counter antagonists, primarily Mayor Humdinger and Harold. However, they do not engage in or win direct close-quarters physical combat against any male opponents.

The film features established characters from the PAW Patrol series, all of whom retain their original genders. New characters introduced also have consistent gender portrayals, with no instances of a character's gender being changed from prior canon.

The film features established animated characters, both human and anthropomorphic animals. There are no instances where a human character, previously established as one race in the PAW Patrol canon, is depicted as a different race in this installment. The concept of race swap does not apply to the animal characters.