Rescue Heroes: The Movie, an animated children's film, does not include any discernible LGBTQ+ characters or themes. The narrative focuses solely on the rescue team's adventures and challenges, with no elements related to queer identity present in the story.

The animated film "Rescue Heroes: The Movie" does not feature any identifiable transsexual characters or themes. Its narrative focuses on a team of rescue workers responding to disasters, without exploring gender identity topics.

The film focuses on the Rescue Heroes team, including female members, engaging in rescue operations and disaster management. The challenges faced are primarily environmental disasters, not human antagonists requiring direct physical combat. No female character is depicted defeating male opponents in close-quarters physical fights.

The film is a continuation of the animated series, featuring the same core team of characters. All established characters from the source material maintain their original genders in the movie. No existing character was portrayed as a different gender.

Rescue Heroes: The Movie is a direct continuation of the animated series. The established races of its characters remained consistent with their original depictions, with no instances of a character's race being altered from their prior canon.