Resident Evil: Vendetta does not feature any identifiable LGBTQ+ characters or themes. The story is solely centered on its established protagonists battling a new bioweapon threat, resulting in no portrayal of queer identity within the film's narrative.

Resident Evil: Vendetta does not feature any identifiable transsexual characters or themes. The film's narrative is entirely focused on action, horror, and the fight against bioterrorism, with no elements related to transgender identity.

The film features Rebecca Chambers, who is not involved in combat, and Maria Gomez, an antagonist. Maria's combat effectiveness stems from her use of firearms and her enhanced abilities as a bio-organic weapon, which are excluded from the criteria for physical combat feats.

The film features established Resident Evil characters like Leon S. Kennedy, Chris Redfield, and Rebecca Chambers, all of whom retain their canonical genders from previous installments and source material. No established character undergoes a gender change.

This animated film features established characters like Leon S. Kennedy, Chris Redfield, and Rebecca Chambers, all of whom retain their canonically established racial depictions from the game series. No character's race was altered from their source material.