Rio 2 does not feature any identifiable LGBTQ+ characters, relationships, or themes. The narrative centers on a heterosexual macaw family and their adventures, resulting in no portrayal of queer identity within the film's storyline.

Rio 2 is an animated family film centered on the adventures of a macaw family in the Amazon rainforest. The narrative focuses on themes of environmental conservation, family dynamics, and cultural differences among birds. There are no identifiable transsexual characters or themes present in the film's storyline.

The film features female characters like Jewel and Gabi, who participate in action sequences. However, their actions against male opponents (loggers, Nigel) involve animalistic defense, sabotage, or the use of unique biological traits (poison), rather than direct physical combat based on skill, strength, or martial arts.

Rio 2 is a direct sequel featuring established characters who maintain their original genders. All new characters introduced in this film do not have prior canonical genders to be swapped from.

Rio 2 features anthropomorphic bird characters. The concept of human race, and therefore a 'race swap' as defined, does not apply to these animal characters.