Rurouni Kenshin Part I: Origins does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on historical action and character development without incorporating queer identities or storylines.

Rurouni Kenshin Part I: Origins does not feature any identifiable transsexual characters or themes. The film's plot focuses on historical samurai action and character development, without engaging with transgender identity in any capacity.

The film features Kaoru Kamiya, a skilled martial artist, who engages in combat against male opponents. However, in these encounters, she is either disarmed without a fight or ultimately overwhelmed and captured, not achieving a clear victory over any male character in direct physical combat.

The film is a faithful adaptation of the original manga, with all major and supporting characters retaining their established genders from the source material. No character canonically or widely known as one gender is portrayed as a different gender in the film.

The film is a live-action adaptation of a Japanese manga set in Meiji-era Japan. All major characters, originally depicted as Japanese, are portrayed by Japanese actors, aligning with the source material and historical context.