Smurfs: The Lost Village is an animated children's film that does not include any discernible LGBTQ+ characters or themes. The narrative focuses on adventure and community among the Smurfs, without exploring queer identities or relationships.

This animated film does not feature any identifiable transsexual characters or themes. The narrative focuses on the Smurfs' adventure to discover a lost village and Smurfette's journey of self-discovery, without engaging with gender identity beyond cisgender portrayals.

The film features female Smurf characters, including Smurfette and the Smurfs of the Lost Village. While they participate in action sequences and evade antagonists like Gargamel, Azrael, and Monty, their victories are achieved through cleverness, evasion, or ranged attacks (archery). There are no instances where a female character defeats one or more male opponents in direct physical, close-quarters combat.

The film introduces a new village of female Smurfs, but these are original characters created for this specific movie's plot. No previously established male or female Smurf character from the wider canon has their gender changed.

The Smurfs are a fictional, blue species and do not possess human racial characteristics. The human character, Gargamel, is consistently depicted as white. No established character's race was changed from prior canon or source material.