Spirit Untamed is an animated adventure film centered on themes of friendship, family, and connection with nature. The narrative does not include any discernible LGBTQ+ characters, relationships, or storylines, resulting in no direct portrayal or impact on queer representation.

Spirit Untamed does not feature any identifiable transsexual characters or themes. The narrative centers on a young girl's adventures with a wild horse, focusing on themes of courage, family, and friendship, without engaging with transgender identity in any capacity.

The film features young female protagonists who engage in adventures to protect wild horses from male wranglers. Their victories are achieved through horsemanship, strategy, and outsmarting their opponents, rather than direct physical combat or martial arts against male characters.

Spirit Untamed is a spin-off of the Spirit Riding Free series, which itself is based on Spirit: Stallion of the Cimarron. All major characters, including Spirit and the human protagonists, maintain their established genders from prior installments or were new creations with consistent gender portrayals.

The film's main characters, including Lucky, Pru, and Abigail, maintain their established racial depictions from the 'Spirit Riding Free' animated series, which serves as the direct source material. No character originally established as one race is portrayed as a different race.