The film "The Soul of Nigger Charley" does not feature any identifiable LGBTQ+ characters or themes. Its narrative focuses on Western action and racial themes, with no elements related to queer identity present in the plot or character arcs.

The film "The Soul of Nigger Charley" does not feature any identifiable transsexual characters or themes. Consequently, there is no portrayal to evaluate regarding transgender identity or experiences within the narrative.

The film primarily focuses on the male protagonists' struggles and combat. Female characters, including Elena, are present but do not engage in or win close-quarters physical combat against male opponents. Their roles do not involve direct physical confrontation or victory in such scenarios.

This film is a sequel to an original story, not an adaptation of pre-existing material with established character genders. There are no instances of characters from the prior film or any historical figures being portrayed with a different gender.

The film features characters, including the titular Charley, who are established as Black within the film's own narrative and genre. There is no evidence of any character being canonically or historically established as a different race prior to this film's production or its predecessor.