Ace Ventura: Pet Detective features its primary antagonist, Lieutenant Lois Einhorn, who is revealed to be Ray Finkle, a cisgender man living as a woman. This revelation is played for extreme shock and disgust, with the protagonist reacting with revulsion. The film uses this identity as a source of mockery and villainy, reinforcing harmful stereotypes and presenting the character's identity as inherently repulsive. The net impact is negative.

The film features a transsexual character whose identity is revealed as a plot twist, primarily serving to shock and disgust the protagonist and audience. This portrayal links trans identity with villainy and deception, contributing to harmful stereotypes and ridicule without counterbalance or critique.