American Horror Stories frequently includes LGBTQ+ characters across its anthology episodes. While these characters often encounter tragic or violent ends typical of the horror genre, their queer identity is generally presented as a characteristic rather than the explicit cause of their suffering or a subject of ridicule. The portrayal offers representation without strong affirmation or denigration of queer identity itself.

American Horror Stories features trans characters across episodes. While one character's identity is incidental, another trans character is subjected to extreme persecution and ultimately killed by a prejudiced community, with her identity being a direct catalyst for her suffering. This results in a net negative impact due to the character's unrelieved misery and punitive outcome.

American Horror Stories frequently portrays Christian institutions and adherents in a negative light, often highlighting hypocrisy, fanaticism, and oppressive behaviors. The narrative uses Christian themes to explore dark aspects of human nature and societal failings, rather than affirming the faith.