Chilling Adventures of Sabrina offers a predominantly positive portrayal of LGBTQ+ characters. Ambrose Spellman's pansexuality is normalized and respected, while Theo Putnam's transgender journey is depicted with sensitivity and strong support. The show integrates diverse queer identities into its narrative, presenting them with dignity, complexity, and agency, contributing to an affirming overall impact.

Chilling Adventures of Sabrina features Theo Putnam, a trans boy whose journey of self-discovery and coming out is depicted with significant affirmation. His identity is treated with respect, and he receives support from his friends and family. The portrayal emphasizes his growth, confidence, and agency, contributing positively to the show's diverse cast.

Ambrose Spellman, a character depicted as white in the source comics, is portrayed by a Black actor in the series. Rosalind Walker, a character with a white comic book counterpart, is also portrayed by a Black actress.

The series consistently portrays Christian institutions and many of its adherents as hypocritical, judgmental, and oppressive, particularly in their persecution of witches. The narrative frequently critiques its dogma and positions it as a restrictive force.