The show features multiple prominent LGBTQ+ characters, including a central gay teenage romance and a genderfluid character whose identity is accepted. While set in a horror context, the series consistently portrays these identities with dignity, complexity, and agency, framing obstacles as external rather than inherent to their queer identity. The overall impact is affirming and validating.

The "Chucky" series features prominent genderfluid and trans male characters whose identities are depicted with respect and affirmation. These characters are given complexity and agency, and their gender identity is not a source of mockery or villainy, contributing to a net positive portrayal.

The characters Glen and Glenda, originally introduced in "Seed of Chucky" as male and female personalities within one body, are portrayed as two separate, explicitly non-binary individuals in the 2021 show. This shift from binary to non-binary representation for established characters constitutes a gender swap.

The show portrays Christian institutions (like the Catholic school) as vulnerable and ineffective against evil, and some Christian characters (like Bree) as hypocritical or superficial in their faith, using it as a coping mechanism rather than a source of genuine virtue. The narrative often satirizes the performative aspects of faith without offering significant counterbalancing positive portrayals.