The Crowded Room offers a unique and introspective exploration of LGBTQ+ themes through a character with dissociative identity disorder. It highlights the fluidity of identity and acceptance, particularly in a relationship where an alter is fully embraced. Despite a controversial scene, the show's overall portrayal facilitates nuanced conversations about queerness, affirming the worth of diverse identities.

The character Arianna, an alter of the canonically male Danny Sullivan, is portrayed as female. This on-screen gender differs from the original canonical gender of the character, fulfilling the definition of a gender swap.

The show thematically engages with Christian concepts of justice and grace, applying them to mental health and crime in a nuanced manner that aligns with the faith's virtues.