The OA features prominent LGBTQ+ characters, including a transgender boy and a gay man, whose identities are portrayed with dignity, complexity, and empathy. The narrative consistently affirms their worth and agency, integrating their experiences respectfully into the overarching themes of identity, connection, and belonging.

The OA features Buck Vu, a trans boy, as a main character whose identity is portrayed with dignity and complexity. His trans identity is integrated naturally into his character arc, without being sensationalized or used for negative stereotypes. The narrative respectfully affirms his worth and agency within the story.

The film respectfully uses Christian concepts like "angels" and "heaven" as a framework for its unique spiritual narrative, often reinterpreting them. Characters with Christian backgrounds are portrayed sympathetically, and the show explores themes of faith and belief without condemning the Christian path.