Tokyo Vice features LGBTQ+ characters, notably Samantha Porter, whose bisexuality is explored through a significant relationship with a woman in Season 2. The series portrays this relationship and other queer individuals within Tokyo's nightlife with dignity and complexity, integrating them respectfully into the narrative without relying on harmful stereotypes or making their identity the sole source of their struggles.

Tokyo Vice includes Akira, a transgender hostess, as a recurring character in its depiction of Tokyo's nightlife. Her trans identity is acknowledged but not central to the main narrative, which focuses more on her professional life and interactions. The portrayal is largely observational, avoiding strong positive affirmation or negative stereotyping, thus presenting her identity incidentally within the show's gritty setting.

The series portrays the protagonist, Jake Adelstein, as Jewish, with his background subtly informing his moral compass and outsider perspective in Japan. His Jewish identity is treated with respect and nuance, serving as a foundation for his character without being subjected to critique or ridicule.