Ace Ventura: When Nature Calls does not feature any identifiable LGBTQ+ characters or themes. The film's plot and character arcs are entirely unrelated to queer identity, focusing instead on a pet detective's comedic adventures in the African jungle.

The film "Ace Ventura: When Nature Calls" does not feature any identifiable transsexual characters or themes. Therefore, there is no portrayal to evaluate, resulting in a 'N/A' assessment for its depiction of transsexual individuals.

The film does not feature any significant female characters engaging in direct physical combat against male opponents where they are shown to be victorious. Female characters primarily serve supporting or comedic roles without participating in such action sequences.

Ace Ventura: When Nature Calls is a sequel featuring original characters from the film series. There is no evidence of any character being established as a different gender in prior source material or history and then portrayed as a different gender in this film.

This film is a sequel where the main character, Ace Ventura, is consistently portrayed by the same actor. All other significant characters are original to this installment, without prior canonical racial depictions to be altered.