An American Tail: The Mystery of the Night Monster does not feature any identifiable LGBTQ+ characters or themes. The film's narrative focuses on Fievel Mousekewitz's adventures and the mystery of a supposed monster, without incorporating elements related to queer identity or experiences.

The film "An American Tail: The Mystery of the Night Monster" does not feature any identifiable transsexual characters or themes. The narrative focuses on Fievel and his friends investigating a mysterious creature, with no elements related to gender identity or transition present in the story.

The film does not feature any female characters engaging in or winning direct physical combat against male opponents. The narrative focuses on adventure and problem-solving, with characters typically outsmarting or escaping threats rather than engaging in close-quarters fights.

This film is a direct-to-video sequel in an established animated franchise. All returning main characters maintain their previously established genders from earlier installments. No new characters are introduced with a gender different from a prior canonical version.

The film features anthropomorphic mouse characters, not human characters. The concept of a 'race swap' as defined, which applies to human racial categories, does not apply to these animal characters.