The film does not feature any identifiable LGBTQ+ characters or themes. Its narrative is centered on absurdist humor and the bizarre exploits of its main characters, without engaging with LGBTQ+ representation.

There is not enough information available to assess the film's portrayal of transsexual characters or themes. Without specific plot details or character descriptions, it is impossible to determine if any transsexual individuals are depicted or to evaluate the nature of such portrayals.

The film features very few female characters, and none are depicted engaging in or winning direct physical combat against male opponents. The robot assistant character is either a victim of experiments or a giant monster controlled by a male character, ultimately defeated by male protagonists.

The film features the established characters from the Aqua Teen Hunger Force series, all of whom maintain their original canonical genders as portrayed in the source material. No characters established as one gender were depicted as a different gender in this film.

The film is a continuation of the animated series, featuring the original voice cast for all established characters. No character who was canonically established as one race in the source material is portrayed as a different race in the movie.