Based on available information, there is no depiction of LGBTQ+ characters or themes in 'Teenage Mutant Ninja Turtles: Chrome Alone 2 - Lost in New Jersey, 2025'. The film's plot focuses on the Turtles confronting a toy company and its anti-AI messaging, with no mention of LGBTQ+ content in any provided details.

Teenage Mutant Ninja Turtles: Chrome Alone 2 - Lost in New Jersey does not feature any transsexual characters or themes. The story focuses on intellectual property theft and an anti-AI message, with no content related to gender identity or transformation.

Based on available information, no combat scenes depict female characters engaging in or winning direct physical combat against male opponents. Therefore, the film does not contain instances of female characters defeating male opponents in close-quarters physical combat.

The provided information, including plot details and voice cast, does not indicate any characters whose gender differs from their established source material. The gender analysis explicitly states no on-screen gender differences were identified.

The film's major characters are anthropomorphic mutant turtles and a robot, none of whom are human. Their on-screen depiction as non-human entities is consistent with their source material, therefore no race swap occurs.