Disaster Movie features brief depictions of characters that rely on harmful LGBTQ+ stereotypes for crude comedic effect. These portrayals are primarily used for mockery, presenting effeminate or gay identities as sources of ridicule without any positive counterbalance or critical perspective.

Disaster Movie features a character parodying Carrie Bradshaw, played by a male actor in a dress. The portrayal relies on the 'man in a dress' trope, using the character's gender non-conforming appearance as a primary source of physical comedy and ridicule. This approach contributes to a negative portrayal by mocking gender presentation rather than offering any nuanced or respectful depiction.

The film features a parody of Prince Caspian, a character canonically established as male in the Narnia series, portrayed by a female actress.

The film features multiple instances where characters widely established as one race are portrayed by actors of a different race. Notable examples include Indiana Jones, Michael Jackson, Silas from 'The Da Vinci Code,' and The Rock.