Dragon Ball: Mystical Adventure does not feature any explicit LGBTQ+ characters, relationships, or themes. The story focuses on the adventures of Goku and his friends, with all depicted identities and romantic interests conforming to heterosexual norms or remaining platonic, leading to no discernible LGBTQ+ portrayal.

The film does not feature any identifiable transsexual characters or themes. While the character Oolong can shapeshift into various forms, including women, this ability is used for disguise and comedic effect rather than as an exploration or expression of gender identity.

The film features Launch, a female character who participates in combat against male opponents. However, her victories are consistently achieved through the use of firearms, which falls outside the defined criteria for physical combat feats.

The film "Dragon Ball: Mystical Adventure" adapts characters from the established Dragon Ball manga and anime. All major characters, such as Goku, Bulma, Krillin, and Master Roshi, retain their canonical genders from the source material in this movie.

This animated film is a direct adaptation of the original Dragon Ball manga. The characters' visual depictions in the movie are consistent with their established appearances in the source material, with no changes to their race.