The film 'Penguins of Madagascar' does not include any discernible LGBTQ+ characters or themes. Its narrative is entirely devoid of such portrayals, leading to a net impact rating of N/A.

The film "Penguins of Madagascar" does not feature any identifiable transsexual characters or themes. The narrative focuses on the adventures of the titular penguins and their mission, without engaging with transgender identity or related issues.

The main characters, Skipper, Kowalski, Rico, and Private, maintain their established male genders from previous installments. New characters introduced in the film do not constitute gender swaps as they lack prior canonical gender.

The main characters are animated penguins, to whom the concept of human race does not apply. There are no instances of human characters from prior canon being portrayed as a different race in this film.