The Trident (2023) does not feature any LGBTQ+ characters, relationships, or themes within its narrative. The film, a short animated adventure in the Puss in Boots universe, focuses on action, humor, and the consequences of bravado, without engaging with issues of sexual orientation or gender identity.

The animated short film "The Trident" does not feature any transsexual characters or address trans themes. Its narrative centers on Puss in Boots recounting a past adventure, consistent with the fantasy and comedy elements of the Puss in Boots franchise, without engaging with gender identity topics.

Based on the provided information, there are no available details describing specific combat scenes in 'The Trident' where female characters defeat male opponents in close-quarters physical combat. Therefore, an assessment of such portrayals cannot be made.

The film's characters, including Puss in Boots, Kitty Softpaws, and Goldilocks, maintain their established genders from source material and previous adaptations, with no on-screen gender changes from their original canonical portrayals.

The film primarily features anthropomorphic animal characters, for whom the concept of human race is not applicable. The only character with a human ethnic origin from source material, Goldilocks, is cast consistently with her traditional European depiction. No established human character's race was changed.