The Unholy Trinity focuses on traditional Western themes of revenge and justice. Available information indicates a complete absence of LGBTQ+ characters or themes, suggesting no representation or related social issues are addressed within the film's narrative.

Based on the provided information, there is no available data to evaluate the portrayal of transsexual characters or themes in 'The Unholy Trinity'. Consequently, no assessment of positive, negative, or neutral impact can be made.

The film features female characters such as Running Cub and Julia, who are influential in the narrative. However, the available information indicates that the film does not portray these or other female characters as fighters or warriors who engage in and win direct physical combat against male opponents.

The provided information explicitly states there is no indication of gender-swapped characters. Main characters like Henry Broadway, Sheriff Gabriel Dove, and St. Christopher are portrayed as male, consistent with typical characterizations, and no gender swapping is noted for any roles.

The film introduces original characters, such as St. Christopher and Running Cub, whose racial identities are integral to its unique narrative set in 19th-century Montana. There is no indication these characters were established with a different race in prior source material or history.