Devious Maids features Adrian Powell, a prominent bisexual character whose sexuality is explored through secret affairs and its impact on his marriage, adding complexity to his often-villainous persona. While his bisexuality is frequently tied to dramatic and scandalous plotlines, it is not consistently depicted as a source of mockery or degradation. Other LGBTQ+ characters appear in more incidental roles, contributing to a portrayal that is present but largely avoids strong positive or negative arcs.

Devious Maids features Daniela Mercado, a transgender character whose identity becomes a central plot point for her mother, Carmen. The show depicts Carmen's initial struggle with acceptance, followed by a resolution of unconditional love and support, portraying Daniela with dignity and agency.

The show consistently portrays Christianity, particularly Catholicism, as a source of comfort, moral guidance, and cultural identity for its main characters. While individual characters may exhibit flaws or hypocrisy, the narrative generally treats the faith itself with respect, often showing characters turning to prayer and spiritual reflection in times of need.