Disenchantment features a positive portrayal of LGBTQ+ themes, primarily through its bisexual protagonist, Princess Bean. Her significant and affirming romantic relationship with Mora the mermaid is depicted with dignity and complexity, serving as a central emotional arc. The show validates queer identity and love without resorting to harmful stereotypes or ridicule.

The show features Princess Bean, who engages in and wins close-quarters physical fights against male guards using a sword and hand-to-hand combat. Queen Oona also demonstrates combat proficiency, defeating male opponents with her unique physical abilities and agility.

The show consistently satirizes its fictionalized medieval Church, portraying its leaders and institutions as corrupt, hypocritical, and power-hungry. The narrative frequently highlights the oppressive nature of religious dogma and the abuse of power by its adherents, without offering significant counterbalancing positive portrayals of the faith itself.