The show features a character living as a man to escape an abusive past, whose biological sex is revealed. The protagonist, Dr. Quinn, responds with empathy and support, while the narrative frames societal prejudice as an external challenge. This portrayal, for its time, was progressive and affirmed the character's dignity and choices.

The show generally portrays Christianity as a positive force for community, morality, and compassion, often through the character of Reverend Johnson. While it critiques the hypocrisy and prejudice of some adherents, the narrative consistently frames these as individual failings, emphasizing the inclusive and loving aspects of Christian teachings.

Judaism is portrayed positively through Myra Bing, showcasing its rich traditions and family values. The narrative consistently condemns the anti-Semitism and prejudice she encounters, positioning the audience to sympathize with her and her faith.