JoJo's Bizarre Adventure is widely celebrated for its strong queer-coding, flamboyant aesthetics, and intense, often intimate bonds between same-sex characters. While explicit representation is limited, the series' embrace of gender non-conformity and its portrayal of deep, implied same-sex relationships, even among villains, contributes to a net positive and validating impact for many LGBTQ+ viewers.

The show features multiple female characters who engage in and win close-quarters physical combat against male opponents. Examples include Lisa Lisa using Ripple-infused techniques and Jolyne Cujoh and Ermes Costello utilizing their Stands, which manifest as physical entities or directly enhance their physical capabilities for direct confrontation.

The film features Father Pucci, a Catholic priest and primary antagonist, whose villainous quest to achieve 'Heaven' involves mass destruction and the manipulation of religious concepts. His actions and ideology are portrayed as fundamentally evil and destructive, with no significant counterbalancing positive depiction of Christianity or its adherents within the narrative.